Targeted isolation of biocontrol agents from plants through phytopathogen co-culture and pathogen enrichment

Abstract In a long-term symbiotic relationship between plants and pathogens, have evolved to harbor beneficially endophytic microbiomes, thereby conferring them the ability resist infection by pathogens. This prompted us establish phytopathogen-based co-culture platform for targeted isolation of potential biocontrol agents from via specific pathogen enrichment. this study, we investigated three different phytopathogenic systems, including kiwifruit, turfgrass, rice, their infectious bacterial and/or fungal By using developed mono- or co-enrichment platform, efficiently isolated antimicrobial agents, Bacillus safensis ZK-1 against Pseudomonas syringae pv. actinidiae that causes kiwifruit canker, alcaligenes ZK-2 Clarireedia paspali dollar spot disease in velezensis ZK-3 rice blight Xanthomonas oryzae blast fungus Magnaporthe . We believe enrichment here is versatile effective with broad-spectrum activities interest large-scale manner.